BIOTECHNOLOGICAL POTENTIAL OF FUSARIUM STRAINS IN BIOPIGMENT PRODUCTION
DOI:
https://doi.org/10.54899/dcs.v22i85.3860Palavras-chave:
Filamentous Mushroom, Microbial Polyketides, Pigment, BicaverinaResumo
Microorganisms are promising sources of biopigments, many of them with antioxidant, antimicrobial, anticancer and antimutagenic properties, and can be applied in food, pharmaceutical, and textile industries, among others. Among them, bicaverine, which is produced by Fusarium species and which has proven antitumor and antimicrobial properties. The objective of this work was to evaluate different strains of Fusarium oxysporum species in the production of this polycaferid and to analyze the influence of variables such as agitation, temperature and pH on the production of biomass, intracellular and extracellular bicaverin. For this, three strains of this fungus were tested: UCP 1624, UCP 1137 and UCP 0119. The results revealed that all species were able to produce pigment in both solid and liquid mediums, but with different yields. Due to obtaining a higher yield of biomass and bicaverine, the species Fusarium oxysporum UCP 1624 was subjected to a complete factorial design 23 that showed that high levels of agitation significantly influence all dependent variables: biomass production and extracellular and intracellular production of bicaverine. However, high pH levels showed a negative effect, indicating that low values contribute to the production of pigment and biomass. To confirm the presence of bicaherin, Infrared Spectroscopy (FT-IR) was performed, which demonstrated the presence of quinones, oxygenated polycyclic aromatic hydrocarbons, as well as the pigment bicaverin, which has in its molecule an oxygen interposed between two carbon atoms (C-O-C) in aromatic chains (benzoxanthone).
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