THE EFFECTIVENESS OF A SUSTAINABLE SILVER NANOPARTICLE PRODUCT IN STOPPING CARIES IN CHILDREN

Autores

  • Bruno Barros de Albuquerque
  • Thaysa Gomes Ferreira Tenório dos Santos
  • Manuella Azevedo Varjal Carneiro Leão
  • Paula Esther Alves Cruz
  • Karina Silva Soares
  • Adriana Stone dos Santos
  • Débora Heloísa Silva de Brito
  • Juliane Rolim de Lâvor
  • Mônica Vilela Heimer
  • Arnaldo Caldas de França França Junior
  • Aronita Rosenblatt

DOI:

https://doi.org/10.54899/dcs.v22i82.3092

Palavras-chave:

Preventive Dentistry, Silver, Dental Caries, Children

Resumo

Objective: This study aims to evaluate caries arrest properties of a liquid solution containing a natural colloidal mineral compound with 40 ppm pure silver nanoparticles (AgNP) as a mouthwash in school-age children. This product is available in Brazil, the United States, Europe, Asia, and Oceania for its claimed germicidal action against pathogenic organisms, including gastrointestinal problems, skin wounds, and respiratory conditions; however, it is not for treating dental infections. Method: This randomized, double-masked clinical trial was conducted in a public school in the city of Recife, Brazil. The sample consisted of 104 children aged 6 to 9 years of both sexes. The chosen product was Almacura®️ brand Colloidal Silver. A group of calibrated dentists from the University of Pernambuco performed a dental inspection and recorded the dmft and DMFT index of the children. After the baseline evaluation, the children were randomly assigned into two groups. In Group 1 (intervention), children rinsed their teeth with the experimental solution. Children in Group 2 (control) brushed their teeth only with fluoridated toothpaste. Children had supplies of toothbrushes and fluoridated toothpaste. The first follow-up exam, assessing caries progress, was at a three-month interval. Arrested caries were those whose activity ceased within three months. Result: This study indicated that the AgNP solution mouthwash arrested carious lesions on its own within three months. The analytical statistics revealed a significant association between caries arrest and the use of the product (P < 0.05). However, the efficacy indicated an NNT of 6, meaning that six children need to be treated to prevent caries in one child.  Conclusion: An AgNP product already marketed in several countries may serve as a coadjuvant method to prevent dental decay in children, to be used in conjunction with traditional methods, thereby increasing their effectiveness in combating dental disease. The product is low-cost and easy to apply.

Downloads

Não há dados estatísticos.

Referências

1. Tinanoff N et al. Early childhood caries epidemiology, aetiology, risk assessment, societal burden, management, education, and policy: Global perspective. Int J Paediatric Dent. 2019;29(3):238-248.

2. Pitts NB, Baez R, Diaz-Guallory C. Understanding dental caries as a non-communicable disease. British Dental Journal, 2021; 231(12): 749-753.

3. Ruff RR, Niederman R. Silver diamine fluoride versus therapeutic sealants for the arrest and prevention of dental caries in low-income minority children: study protocol for a cluster randomized controlled trial. Trials, 2018;19:1-9.

4. Nishino M, Yoshida S, Sobue S, Kato J, Nishida M. Effect of topically applied ammoniacal silver fluoride on dental caries in children. J Osaka Univ Dent Sch, 1969; 9:149-155

5. Rosenblatt A, Stamford TC, Niederman R. Silver Diamine Fluoride: a caries “silver fluoride bullet”. J Dent Res, 2009;88(2):116-25

6. Targino AGR, Flores MAP, Junior VES, Bezerra FGB, Freira HL, Galembeck A et al. An innovative approach to treating dental decay in children. A new anti-caries agent. Journal of Materials Science: Materials in Medicine, 2014;25:2041-2047

7. Arnaud M , Junior PCM, Lima MG , Silva AVE , Araujo JT , Gallembeck A et al. Nano-silver fluoride at higher concentration for caries arrest in primary molars: A randomized controlled trial. International Journal of Clinical Pediatric Dentistry, 2021; 14(2):207-211.

8. S3 NANO. Retrieved from: https://s3nano.com/ [Accessed: july 10, 2024].

9. Domínguez AV, Algaba RA, Canturri AM, Villodres ÁR, Smani Y. Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria. Antibiotics, 2020; 19;9(1):1-10

10. Dhapte V, Kadam S, Moghe A, Pokharkar V. Probing the wound healing potential of biogenic silver. Journal of Wound Care, 2014;23(9): 431-436

11. Ovais M, Ahmad I, Khalil AT, Mukherjee S, Javed R , Ayaz M et al. Wound healing applications of biogenic colloidal silver and gold nanoparticles: recent trends and future prospects. Applied Microbiology and biotechnology, 2018;102(10):4305-4318

12. Gruebbel AO. A measurement of dental caries prevalence and treatment service for deciduous teeth. Journal of Dental Research, 1944; 23(3):163-168

13. Klein H, Palmer CE. Dental caries in American Indian children. US Government Printing Office; 1938. 54p

14. Silva AVC, Teixeira JA, Júnior PCM, Lima MGS, Mota CCBO,Lins ECCC et al. Remineralizing potential of nano-silver-fluoride for tooth enamel: an optical coherence tomography analysis. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2019; 19(1):1-13

15. Ladewig NM, Sahiara CS, Yoshioka L, Olegário IC, Floriano I, Tedesco TK. Efficacy of conventional treatment with composite resin and atraumatic restorative treatment in posterior deciduous teeth: study protocol for a randomized clinical trial. BMJ, 2017; 7(7):1-6

16. Tirupathi S, Svsg N , Rajasekhar S, Nuvvula S . Comparative cariostatic efficacy of a novel Nano-silver fluoride varnish with 38% silver diamine fluoride varnish a double-blind randomized clinical trial. Journal of clinical and experimental dentistry, 2019; 11(2): e105-112

17. Santos Jr VES, Filho VA, Targino AG, Flores MA, Galembeck A, Caldas AF Jr, et al. A new "silver-bullet" to treat caries in children--nano silver fluoride: a randomised clinical trial. J Dent. 2014; 42(8): 945-951

18. Waikhom N, Agarwal N, Jabin Z, Anand A. Antimicrobial effectiveness of Nano Silver Fluoride Varnish in reducing Streptococcus mutans in saliva and plaque biofilm when compared with Chlorhexidine and Sodium Fluoride Varnishes. Journal of Clinical and Experimental Dentistry, 2022; 14(4):e321-e328

19. Hsiaoi L, Hsieh YK, Wang CF, Chen IC, Huang YJ. Trojan-horse mechanism in the cellular uptake of silver nanoparticles verified by direct intra- and extracellular silver speciation analysis. Eviron Sci Technol, 2015; 49(6): 3813–3821

20. Besinis A, Peralta T, Útil RD. The antibacterial effects of silver , titanium dioxide and sílica dioxide nanoparticles compared to the dental disinfectant chlorhexidine on Streptococcus mutans using a suíte of bioassay. Nanotoxicology. 2014;8(1):1-16

21. Freire PLL, Albuquerque AJR, Farias IAP, Silva TG, Aguiar JS, Galembeck A et al. Antimicrobial and cytotoxicity evaluation of Colloidal Chitosan − Silver nanoparticles − Fluoride nanocomposites. International Journal of Biological Macromolecules, 2016; 52: 896-903

22. Freire PLL, Stamford TCM, Albuquerque AJR, Sampaio FC, Cavalcante HMM, Macedo R. Action of silver nanoparticles towards biological systems: cytotoxicity evaluation using hen's egg test and inhibition of Streptococcus mutans biofilm formation. International Journal of Antimicrobial Agents (Print), 2015; 45: 183-187

23. Scarpelli BB, Punhagui MF, Hoeppner MG, Almeida RSC, Juliani FA, Guiraldo RD et al. In vitro evaluation of the remineralizing potential and antimicrobial activity of a cariostatic agent with silver nanoparticles. Brazilian dental journal. 2017; 28: 738-743

24. Lâvor JR, Abreu RSA, Costa SGA, Brito DHS, Santos TGFT, Almeida NKVL et al. Efficacy of a high concentration of silver nanofluoride in preventing caries: a randomized clinical trial. J Clin Exp Dent., 2014; 16(3);e263-269

25. Santos Jr VE., Targino, AGR, Flores MA P, Pessoa H de LF, Galembeck A, Rosenblatt A. Antimicrobial activity of silver nanoparticles in treating dental caries. Revista Da Faculdade De Odontologia – UPF, 2014; 18(3).

26. Baker C, Pradhan A, Pakstis L, Pochan DJ, Shan SI. Synthesis and antibacterial properties of silver nanoparticles. Journal of nanoscience and nanotechnology, 2005; 5 (2): 244-249

27. Noronha V, Amauri P J, Duran G, Galembeck André, Cogo-Muller, Karina F-M, Michelle Duran N. Silver nanoparticles in dentistry. Dental materials, 2017; 33(10): 1110-1126

28. Andrade MCM, Rosenblatt A, Galembeck A. Silver nanoparticles penetration in dentin: implications for long-term caries arrestment. Materialia, 2022; 24:101489

29. Edward-Jones V. The benefits of silver in hygiene, personal care and healthcare. Letters in Applied Microbiology,2009; 49(2): 147-152

30. Porto RV; Paim TM, Soares VM, De Jesus MB. Capacidade citotóxica da prata coloidal em células de fígado. XXX Congresso de Iniciação Científica da UNICAMP, 2022

31. Almacura produtos naturais. Retrieved from: https://www.almacura.com.br/ [Accessed: july 10, 2024]

32. de Lavôr JR, de Abreu RS, Costa SA, et al. Efficacy of a high concentration of nano silver fluoride in preventing caries: A randomized controlled trial. J Clin Exp Dent. 2024;16(3):e263-e269. Published 2024 Mar 1. doi:10.4317/jced.60776

Downloads

Publicado

15-09-2025

Como Citar

Albuquerque, B. B. de, Santos , T. G. F. T. dos, Leão, M. A. V. C., Cruz, P. E. A., Soares, K. S., Santos, A. S. dos, … Rosenblatt, A. (2025). THE EFFECTIVENESS OF A SUSTAINABLE SILVER NANOPARTICLE PRODUCT IN STOPPING CARIES IN CHILDREN. Revista DCS, 22(82), e3092 . https://doi.org/10.54899/dcs.v22i82.3092

Edição

Seção

Artigos